Empty Affinity Chromatography Column – Protein Purification 1–300mL
Empty Affinity Chromatography Column – Precision Protein Purification
Upgrade your laboratory workflow with this Empty Affinity Chromatography Column, engineered for reliable, high-performance protein purification across a wide range of research and biochemistry applications. Designed with medical-grade materials and versatile sizing, this column delivers consistent results from small-scale experiments to large-scale purification runs.
Key Features
- Medical-Grade Polypropylene Column Tube: Excellent biocompatibility and minimal non-specific binding ensure sample integrity throughout the purification process.
- Hydrophilic Ultra-High Molecular Weight Polyethylene Sieve Plate: Provides precise flow rate control with no protein adsorption, preserving your valuable samples.
- Wide Size Range (1 mL to 300 mL): Accommodates purification applications of different scales — from analytical micro-purifications to preparative-scale runs.
- Luer Port Design: Compatible with standard syringes and peristaltic pumps for seamless integration into your existing lab setup.
- 50 μm Hydrophilic Frits: Dual frits included for optimal bed support and consistent flow distribution.
Typical Applications
- Purification of recombinant proteins (His-tag, GST, and other affinity tags)
- Purification of antigens and antibodies
- DNA purification and DNA-binding protein isolation
- Glycoprotein and phosphorylated protein purification
- Removal of mycotoxins such as endotoxins from biological samples
What's Included
- 1 × Empty column tube (long body)
- 1 × Upper cover
- 1 × Lower cover
- 2 × Hydrophilic frits (pore size: 50 μm)
Why Choose This Column?
Whether you're working on His-tag protein purification, antibody isolation, or endotoxin removal, this empty affinity column offers the flexibility to pack your resin of choice while ensuring reproducible, low-contamination results. The long-body design increases bed volume capacity, making it ideal for higher-load purification needs.
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Frequently Asked Questions
Q: What materials are used in this affinity chromatography column?
A: The column tube is constructed from medical-grade polypropylene, offering strong biocompatibility and minimal non-specific binding. The integrated sieve plates are made from hydrophilic ultra-high molecular weight polyethylene, which ensures excellent flow rate control with no protein adsorption — critical for maintaining sample purity.
Q: What sizes are available for this empty affinity chromatography column?
A: This empty affinity chromatography column is available in a range of sizes from 1 mL up to 300 mL, making it suitable for both micro-scale analytical purifications and larger preparative-scale protein purification workflows. The long-body design featured in this listing is ideal for higher bed-volume applications.
Q: What types of resins or media can I pack into this empty column?
A: This column is designed to be packed with your resin or chromatography media of choice, including Ni-NTA (His-tag), Protein A/G, glutathione (GST-tag), ion exchange resins, and other affinity media. The 50 μm hydrophilic frits provide excellent support for most standard resin particle sizes used in laboratory protein purification.
Q: Is the Luer port compatible with standard lab equipment?
A: Yes. The column features a standard Luer port that connects easily to syringes, peristaltic pumps, and FPLC-type systems, ensuring compatibility with common laboratory setups for gravity flow, low-pressure, or pump-assisted chromatography applications.
Production & Packaging Notice
To maintain high stock availability and fast shipping times, our items are sourced from multiple verified manufacturing facilities. While the core quality, materials, and product specifications remain identical, you may notice slight variations in the packaging design, manufacturing origin, or the wording of the printed instructions.